Previous investigations of the longevity of overwintered boll weevils on pre-fruiting cotton have yielded variable results. However, environmental conditions were generally not controlled in these studies. We re-examined the longevity of overwintered boll weevils fed pre-fruiting cotton under controlled conditions. Trap-captured overwintered boll weevils were held at 24±1°C and were supplied water alone, cotyledon stage, or four-leaf stage cotton seedlings. Mean longevities of males and females were similar. Overwintered weevils survived an average of ~7 days on water alone, 81 days on cotyledon stage plants, and 62 days on four-leaf stage plants. Maximum observed longevity was 157 days. Our results indicate substantially longer longevities on pre-fruiting cotton than have been previously reported.