A cross of two strains of Heliothis virescens (tobacco budworm), each breeding pure for a different yellow-eyed mutant (wild type is grey-eyed) gave 6 black-eyed progeny among 500 progeny. From the black-eyed progeny were descended many lines in which several other mutant phenotypes were observed. Exposure to 5µg cypermethrin per vial killed 46.1% in the seventh generation descendants of that cross, with lines D38, D41 and D63 resistant with less than 17% mortality. For the domain Hpy, we found two new Hpy alleles (Hpy51 and Hpy52) in eye mutant lines. Frequency of new alleles was 0.85. There are no new Hpy alleles in D8, D28, and, D29 lines which are susceptible type to cypermethrin. For the domain IIS6 region in recent field tobacco budworm samples (Wadmalaw2000), the frequencies of homozygotes (L/L), heterozygotes (L/H), and homozygotes (H/H) were 0.44, 0, and 0.56 respectively without new Hpy alleles.