For the first time, continuous culture in liquid medium of excised roots of Gossypium hirsutum has been achieved, healthy roots being maintained for at least 4 weeks. Cultures were incubated in a minimal rocking condition in the dark at room temperature. Half-strength MS basal liquid medium plus 50 mg myo-inositol, 0.5 mg Nicotinic acid, 0.5 mg Pyridoxine-HCl, 0.5 mg Thiamine-HCl, and 15 g/L glucose was found to be the best medium [CRCM - Cotton Root Culture Medium] for the culture of excised roots.The elongation of roots was more effective in liquid than on agar medium. Root cultures showed differences with various species and medium. However, the pattern of elongation of root tips of cotton was similar: at the beginning, there was a big surge of root elongation, and then a dramatic slow down after the second week, continuing without death even after 10 weeks. This disjointed pattern of elongation was also seen in uncut, soil-grown plants. It is hoped this method has potential use for the research of nematode infection.