About
  PDF
Full Text
(77 K)

Enhanced Roundup Ready Tolerance and Agronomic Trials – Maricopa, Arizona

Jay Subramani, William B. McCloskey and Hal Moser


ABSTRACT

The trial was planted on 4 May 2001 in Maricopa, AZ. Total area was 2.7 acres. The design was a split plot with 4 replications. The main plot treatments were unsprayed, 1.5 lb ae/A and 2.25 lb ae/A Roundup applications. The sub plot treatments were 10 different transformation events. Plots were sprayed on the 3, 6, 10 and 14 node stages. The number of dead cotton plants counted 10 days after first Roundup application showed that there were significant differences between transformation events. It may be that the genotypes had different levels of contaminated seed or the trait that makes them susceptible. Plant height was found to be significantly different with the 1445 plots at 170 cms being tall and the eRR-3 and eRR-9 plots at 140 cms being short. Aborted bolls resulted in carbohydrates being redirected to plant growth making 1445 tall. There may be genotype differences too. Rates of Roundup had no significant effect on events.

Plants were rated for sterility when most of them were at first bloom (42 days after last Roundup application). Ten white flowers at first position were rated. Rating was done at about weekly intervals. The first 3 ratings done at 8, 15 and 25th day after the last Roundup application showed 1445 sprayed with Roundup to be significantly different from the other events. Unsprayed 1445 plants were fertile. Fourth rating done on the 29th day after the last roundup application showed no significant differences between events tested. The glyphosate might have been metabolized or degraded to low levels between 25th and 29th day after Roundup application.

Plot yield from the tolerance trial showed no significant differences. The 1445 plots might have been able to recover and produce bolls comparable to other events tested. Yields were low and this may be because of climate in Maricopa during July, August. Maximum temperature is about 115 F and nighttime temperature around 85 coupled with low heat tolerance of Coker lines.

The agronomic trial had a randomized block design with 10 events. Five had the glyphosate resistance gene and 5 without. DP 565 was a substitute, yielded the most. ERR-9 negative yielded the least and the differences were significant. ERR-6 was the only line that had reduced agronomic performance that resulted from the inserted gene sequence. There were no significant differences between the other eRR events tested.





[Main TOC] | [TOC] | [TOC by Section] | [Search] | [Help]
Previous Page [Previous] [Next] Next Page

Document last modified May 20, 2002