The reniform nematode (Rotylenchulus reniformis) was collected from a field in continuous cotton production and examined for fungal antagonists. Ten fungi were isolated from the vermiform and adult stages of the reniform nematode. Acremonium sp., Arthrographis sp., Chaetomium aureum, Fusarium equiseti, Pseudorobillarda sp., and 5 unknown genera were isolated. Acremonium sp. and Arthrographis sp. occurred in the highest frequency. In greenhouse pathogenicity tests, Arthrographis sp., Pseudorobillarda sp., Fusarium equiseti, MSA1 and MSA5 significantly reduced reniform nematode population development on cotton.