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Metabolic Engineering for Increased Cottonseed Oleic Acid Content

K. D. Chapman, S. Austin-Brown, H. Wessler, T. Huynh, C. Hoang, S. Sparace, T. Ricchiuti, A. Kinney, K. Ripp, I. Pirtle, R. Pirtle and M. Nampaisansuk


 
ABSTRACT

Transgenic cotton plants were developed with altered seed fatty acid profiles aimed at higher monounsaturate levels, specifically oleic acid. Plants were generated by Agrobacterium-mediated transformation, followed by induction of embryogenic calli on kanamycin selection media. A binary vector was designed to suppress expression of the endogenous cottonseed delta-12 desaturase (FAD2) by subcloning a mutant allele of a rapeseed fad2 gene downstream from a heterologous, seed-specific promoter (phaseolin).Fatty acid profiles of total seed lipids from 48 independent transgenic lines were analyzed by gas chromatography. Twenty-five plants showed elevated levels of seed oleic acid content compared with vector-only transformed controls or non-transformed plants of the Coker 312 background. Increased oleic acid content ranged from 21% to 37% (by weight) of total fatty acid content in pooled seed samples from primary transformants. The increase in oleic acid content was solely at the expense of linoleic acid, consistent with reduced activity of FAD2. Collectively, our results extend the metabolic engineering of vegetable oils to cottonseed, and should provide the basis for the development of a family of novel cottonseed oils.





Reprinted from Proceedings of the 2001 Beltwide Cotton Conferences pp. 1434 - 1436
©National Cotton Council, Memphis TN

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Document last modified XXXXXX, XXX XX 2001