The Favimat, a single fiber-testing machine, was used to quantify affects of cotton crimp on fibers from 3 cotton bales. These 3 bales consisted of cotton containing a low, high, and ideal micronaire for textile processing. In order to get a better representation of all fibers within these bales the cotton was further divided into the Suter-Webb array length groups. Prior to testing, individual cotton fibers were removed from each length group and pre-tensioned with a 50 mg clip in the Favimat. An opto-electrical sensor on the Favimat captured the image of each fiber for crimp analysis prior to testing. Following cotton crimp image capturing, fiber fineness was determined by the vibroscope method. A gauge length of 10 mm and a crosshead speed of 20 mm/min were used in tensile testing. Single fiber Favimat testing proved tedious but did produce comparable fineness, elongation, and tenacity results in a relatively short period of time. Future experimentation with a Favimat robot coupled with modifications in parameters could simplify testing. The mean values for these bales indicate that those varieties containing more crimp in the fiber leads to a larger elongation, force to break, linear density, tenacity, and work to rupture. The 7 length groups from these bales indicate that longer cotton fibers appear to contain more crimp per cm. A comparison of single and bundle fiber results appear to show the same trends with Favimat values generally larger than Stelometer, HVI, and Fibronaire values except for HVI tenacity. Based on a variety of testing procedures, the results suggest that the Favimat appears satisfactory for measuring current and future cotton properties.