The objective of this work was to determine if fungal epizootics in cotton aphid populations could be initiated artificially. Greenhouse-grown cotton plants were infested with cotton aphids, Aphis gossypii. After aphid populations were well established they were infected with the fungus, Neozygites fresenii, causing "mini-epizootics" and placed in a commercial cotton field. Prevalence of the fungus was monitored adjacent to and at various distances from the release sites and compared statistically. Based on the data it appears that fungal epizootics can be initiated by early release of infected aphids on greenhouse-grown cotton plants.