The effects of the nonsteroidal ecdysteroids tebufenozide on some biochemical of the haemolymph Spodoptera littoralis larvae was studied. This include the effects of total protein, lactic acid dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G-6-PDH), malic acid dehydrogenase (MDH), alkaline phosphatase (Alkapase), acid phosphatase (Acpase), aspartate glutamate aminotransferase (GOT), alanine oxaloacetic aminotransferaase (GPT) and food consumption. The data show that tebufenozide have a significant effect on the above mentioned enzyme activities. However, MDH, Alkapase did not affect with tebufenozide. Also, tebufenozide has no effect on Acpase, but has a great effect on GOT and GPT. The relative consumed rate for S. littoralis was decreased as the concentration of tebufenozide increase.