Predaceous arthropods and bollworms (Helicoverpa zea [Boddie]) were monitored throughout the season in large plots of Bollgard and conventional cotton with and without early season disruption of predaceous arthropods with acephate (Orthene®). Cyhalothrin (Karate Z®) was applied as needed for bollworm control in disrupted and non-disrupted conventional plots and spinosad (Tracer®) was applied in a similar manner to Bollgard plots. Spinosad was also applied for bollworm control in nondisrupted conventional plots. Early-season applications of acephate prior to the bollworm flight caused reductions in predator populations that lasted throughout the season, resulting in higher numbers of bollworms and more applications to disrupted plots. Applications of broad-spectrum insecticides prior to the bollworm flight should be avoided. In a related study, predaceous arthropods and bollworms were monitored throughout the season in small plots of conventional, Bollgard, and Bollgard II cotton. No differences were found in predaceous arthropod populations within the different cotton genotypes.