Ten cotton, Gossypium hirsutum L., cultivars or breeding-lines were evaluated in the field for resistance to the cotton leaf crumple (CLCr) disease caused by (Genus Begomovirus, Family Geminiviridae) Cotton leaf crumple virus (CLCrV) transmitted by silverleaf whitefly, Bemisia argentifolii Bellows and Perring. The cultivars Texas 121, AP 4103, AP 6101 and Stoneville 474 and the breeding-lines were NK 2165C, NK 2108SS, NK 2387C, NKX C429-93-2ct, NKX 2907, and NKX 2207 were in Imperial Valley, CA. Cotton entries were rated for severity of CLCr disease symptoms and the presence of CLCrV in leaves of selected plants of each cultivar/breeding-line was determined by dot blot hybridization with a CLCrV DNA probe and PCR analysis with degenerate geminivirus primers. DNA sequencing of geminivirus DNA-A and DNA-B fragments, amplified from symptomatic cotton plants, was used to confirm geminivirus infection and partial characterize CLCrV. Results showed differences in whitefly infestation levels and virus disease symptoms among cotton entries. The cultivar AP 4103 had a higher CLCr disease rating than other entries except AP 6101. The breeding-line NK 2387C, with Cedix parentage, had a lower CLCr disease rating than other entries except Stoneville 474 and NK X2207. There were visible CLCr disease symptoms in Stoneville 474 and NKX 2207, but KN 2387C did not display visible CLCr disease symptoms nor was viral DNA detected in this line.