Eight cotton, Gossypium hirsutum L., cultivars or breeding-lines were evaluated for relative susceptibility to charcoal rot, which is caused by Macrophomina phaseolina (Tassi) Goidanich. The cultivars were Texas 121, AP 4103, AP 6101, and Stoneville 474 and the experimental breeding-lines were DG 2165, DG 2108, DG 2383, and DG 2387. On 26 July, wilting and death of cotton plants was noticed. Macrophomina phaseolina was identified by the presence of the sclerotia on the roots of affected plants. On 30 August 1999, the disease incidence was evaluated within in 4-meter section of each of two rows within each plot. The cotton was hand-harvested from 4-meter long sections on 5 October, and seed cotton and lint weights were taken. Disease incidence was lowest in AP 6101, followed by AP 4103; all other entries preformed similarly, P£0.05. AP 6101 had a higher yield than all other genotypes except for AP4103 and DG 2108. There was a correlation between disease incidence and yield (r= 0.8499: p < 0.01).