Cotton fiber stickiness caused by the presence of sugars can not only depend upon the total amount sugars, but which specific ones are present. Studies were conducted to measure the stickiness potential of seven sugars commonly found in honeydew cottons. A single upland cotton was treated with pre-determined amounts of these sugars by misting with an air brush applicator. Resultant treatments were analyzed for sugar content to determine actual sugar retention. Stickiness measurements were conducted on rotary blended and hand blended treatment samples to determine degrees of stickiness at five different sugar concentrations. The characteristic honeydew sugars trehalulose and melezitose as well as the disaccharides turanose and sucrose were found to exhibit higher stickiness potentials than the other tested sugars on the thermodetector, especially at concentrations above 0.5%. Sucrose treated cottons did not get high ratings on the minicard test as observed for the same sugar on the thermodetector.