A new device to rapidly estimate the stickiness of both seed cotton and lint cotton was patented in 1997. This study further evaluated the new device using 500 reference samples that were evaluated with a standard thermodetector as well as an automated thermodetector (H2SD) at other laboratories. Measurement of the stickiness of cotton is typically accomplished with the thermodetector method which is time-consuming, somewhat objective, and destructive. In this study, samples were classified into level of stickiness from 0 to 3 based on the thermodetector as the reference method. The thermodetectors differed from each other as to level of stickiness about 80% of the time and misclassified the samples as to sticky or non-sticky 22% of the time. The new device correctly identified 76% of the samples as either sticky or non-sticky when compared to either thermodetector.