Bollgard II, Monsanto line 15985, that contains two strains of Bacillus thuringiensis (Cry1A(c) and CryX), was evaluated in field plots to determine efficacy against lepidopterous pests in cotton. Control of both the cotton bollworm, Helicoverpa zea (Boddie) and the soybean looper, Pseudoplusia includens (Walker), was significantly greater in Bollgard II compared with a Bollgard variety (DP50B) of cotton that contains only the Cry1A(c) endotoxin. Use of this new technology should provide growers a very low insecticide environment in cotton production where limited applications are needed to control lepidopterous pests.