Densities of insect and spider predator of cotton pests were sampled and compared on Bt and non-Bt cotton from large acreages on producers fields and from a replicated study on the Texas Tech University Research Farm near New Deal, TX. Insect and spider predators were grouped as those with chewing mouthparts, piercing-sucking mouthparts and the two groups added together for total predators. Some significant differences occurred in the large scale, side-by-side comparisons on producers fields, and the majority of the differences were in favor of higher predator numbers in Bt cotton as opposed to non-Bt cotton. The replicated study resulted in no significant differences in insect or spider predators regardless of how they were grouped. When all samples sites were combined, three piercing-sucking predators, (minute pirate bug, Orius tristicolor, big-eyed bugs, Geocoris punctipes (Say) and the cotton fleahopper Pseudatomoscelis seriatus (Reuter) contained 89 % of the total number of piercing-sucking predators in Bt cotton, and 90 % of those collected in non-Bt cotton. A significant portion of chewing predators came from spiders, Araneae, lady beetles, Cocinellidae, and hooded beetles, Notoxus spp., which accounted for 94 % of the chewing predators in Bt cotton and 96 % in non-Bt cotton. A noticeable trend in predator dynamics was that chewing predators out-numbered the piercing-sucking predators 2 to 1 in the first week of July, while the reverse was true for the second week in August. Total numbers of predators averaged across Bt and non-Bt cotton increased from 55,000 per acre the first week in July to 233,000 by the second week in August. We do not believe there are any differences in the key insect and spider predators inhabiting Bt versus non-Bt cotton. These results are positive in that Bt-cotton may act as a "refuge" for predaceous insects and spiders in large scale cotton production where non-Bt cotton may be sprayed with insecticide.