The 32-channel acoustic system described in this paper replaces the cutting method of detecting pink bollworm in bolls. It is shown that the system can detect pink bollworm even in the first instar. Because the system senses all channels simultaneously, it is extremely rapid. Also it is much less labor intensive and significantly reduces the risk of human error. The system can detect all types of insect infestation. It can be used, for example, to inspect fruit and vegetables entering the United States. In addition to detecting infestation, the system can be used in laboratory research, for non-intrusive monitoring of the life cycle of larvae in agricultural commodities, and other host materials. It can also be used for monitoring larvae in the soil. It can operate continuously without human intervention over periods of days, weeks and even months.