A study was conducted in 1998 and 1999 to determine the effects of dual rates of insecticide, nitrogen, and Pix® on arthropod populations in cotton (Gossypium hirsutum). Sample data was recorded for ten arthropod populations. Populations sampled with a drop cloth included tarnished plant bugs (Lygus lineolaris), big-eyed bugs (Geocoris spp.), lady beetles (Coccinellidae), insidious flower bugs (Orius insidiosus), damsel bugs (Nabis spp.), lacewings (Chrysopidae), spiders (Araneae), beet armyworms (Spodoptera exigua), and tobacco budworms (Heliothis virescens) and cotton bollworms (Helicoverpa zea) (grouped as heliothines). Heliothine larvae and eggs were also counted during the visual samples. A significant positive effect of insecticide application on the number of eggs was found in 1998. Fewer larvae, big-eyed bugs, and total hemipteran predators were found in plots receiving supplemental insecticide applications. In 1999, more lady beetles were found in plots receiving a 100 lb/acre rate of nitrogen while an increase to 150 lb/acre showed no increase in lady beetle populations. Analysis indicated that there were more heliothine larvae (1998), total predators (1998), ants (1998), hemipteran predators (1998-99), and big-eyed bugs (1999) in plots receiving Pix applications.