Plant MYB-type proteins contain DNA-binding domains which regulate both general and specific gene transcription. Two cotton cDNA clones (GhMYB1 and GhMYB2), which encode MYB-like proteins, have been isolated from 15 DPA (days post-anthesis) fiber mRNAs by using 5' and 3' RACEs (rapid amplification of cDNA ends). The derived amino acid sequences from these two full-length fiber myb cDNAs show that the basic N-terminal DNA-binding domains of cotton fiber MYBs are highly homologous to other plant MYB-type proteins. Based on the comparison of cDNA and genomic sequences, each of the two myb genes contains two introns located in the coding region of the gene. The expression patterns of these two myb genes were studied by Northern blotting analysis using total RNA from different cotton tissues. The GhMYB1 and GhMYB2 cDNAs were cloned into an expression vector, pET-32b(+), and the expressed His-MYB fusion proteins were purified and used for DNA-binding studies.