Five TAES cotton (Gossypium hirsutum L.) cultivars were transformed using two vectors, pXBOP and pJMA4, which contained genes associated with plant pathogenesis (PR genes): osmotin (PR-5) and a basic PR-1 in pXBOP, and lox1 in pJMA4. Plants were transformed using the shoot apex/Agrobacterium method (US Pat. # 5,164,310; Smith et al., 1992) developed earlier for cotton (Gould et al., 1991a; 1991b). After kanamycin selection, shoots (T₀) were grafted onto in vitro grown seedling rootstocks (Luo and Gould, 1999). Grafted/regenerated plants were normal, fertile, and produced normal progeny. The transferred genes were detected in the DNA of regenerated plants (T₀) and in progeny (T₁) using PCR amplification and Southern DNA analyses. Gene expression was detected by RT-PCR in the progeny.