Colored lint cotton has been grown and used for centuries. Fiber properties have genetic and environmental components. The first level of fiber property variability is at the seed level. White cotton fibers at the micropylar region of a seed are shorter and more mature than fibers in the chalazal region. A comparison of white cotton fiber properties and seed weights revealed that increases in seed weight were accompanied by increases in fiber length and maturity. At the boll level, white lint cotton properties have been mapped by boll location. Fiber property variability was assessed at the seed level in green lint cotton grown in Texas (BC 1995 selection) and in California (BC 1997 selection). Fiber property variability at the boll level was monitored using Texas-grown cotton.