In the United States, root-knot (race 3), and reniform nematodes are the most economically damaging pests of Upland cotton. Progress in developing root-knot nematode resistant germplasm has occurred; however, development of reniform nematode resistant germplasm has received little attention. This report summarizes the progress of the Weslaco and College Station, TX USDA-ARS efforts. Three new strains, RN96625-1, RN96425 (same as RN96424), and RN96528 (same as RN96527) have shown excellent potential as breeding lines. In addition, when new F₃ nematode selections were grown near to and compared with standard non-nematode selected varieties and breeding lines, soil vermiform populations were similar among entries at 6-12 inch and 18-24 inch depths. At 30-36 inches, vermiform populations of the F₃ selections were generally one-half of the populations measured for the standard cultivars and breeding lines.