ABSTRACT
Our objective is to develop transgenic cottons with innate resistance to invading phytopathogens. Utilizing two gene constructs, encoding either a bacterial haloperoxidase or a synthetic antimicrobial peptide, we have regenerated several cotton plants from multiple transformation experiments. We previously reported that crude leaf extracts from stably transformed tobacco plants, expressing either the haloperoxidase (from Pseudomonas pyrrocinia) or the synthetic antimicrobial peptide, D4E1, showed significant antifungal activity in vitro against Aspergillus flavus, Verticillium dahliae and Fusarium moniliforme. In addition, the transformed tobacco plants were shown to inhibit the growth in planta of a fungal pathogen, Colletotrichum destructivum, that causes anthracnose, and a bacterial phytopathogen, Pseudomonas syringae pv. tabaci that causes wildfire.
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