Tests were performed to clean seed cotton, lint, and lint cleaner waste with cylinder cleaners operated with different types of grid bars and cylinder speeds. Results showed that more aggressive, square grid bars performed better than conventional round grid bars in cleaning all three types of material, but allowed more fiber to escape with the trash when cleaning seed cotton. Increasing cylinder speeds from 480 to 1100 rpm also increased the cleaning performance of the cylinder cleaners. Of the five types of grid bars evaluated in this study, the normal grid bars (3/8 in. round bars with 3/8 in. gaps between the bars) remained the preferred type for seed cotton cleaning. The cylinder cleaner with sharp, square grid bars operated at 1100 rpm cylinder speed cleaned lint better than the other treatments and was 80% as effective as a conventional saw-type lint cleaner. Also, a cylinder cleaner equipped with flat, square grid bars and operated at 1100 rpm appeared to be the best solution to balancing lint cleaner waste cleaning and fiber wastage. These modifications provide additional cleaning and may cause less fiber damage.