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Cotton Callus Tissue Transformed with Glutathione Reductase CDNA Derived Frompisum Sativum L. Exhibit Increased Glutathione Reductase Activity

Stephen W. Banks, Faye E. Gordon, Satyendra N. Rajguru, Tanja J. Minova, Dalton R. Gossett and M. Cran Lucas


 
ABSTRACT

Detailed investigations of the activity profile of antioxidant enzymes in salt stressed cotton plants, cotton callus and ovule culture has focused attention on the possible role of these enzymes in the mechanism of salt tolerance in non-halophytes. The critical role of glutathione reductase in the antioxidant enzyme system in plants has prompted us to advance the hypothesis that an increase in the transcription of glutathione reductase may up-regulate the ascorbate-glutathione cycle and confer an enhanced ability of transformed tissue to tolerate higher levels of salt. In order to test this hypothesis we have genetically transformed cotton callus with a cDNA encoding glutathione reductase derived from pea plants. Transformed calli show an increase in glutathione reductase activity of between 128-330% over controls. We are currently examining the growth performance of this tissue on salt amended growth media.



Reprinted from Proceedings of the 1999 Beltwide Cotton Conferences pp. 542 - 546
©National Cotton Council, Memphis TN

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Document last modified Monday, Jun 21 1999