This study employed fibers taken from 25DPA bolls collected at 7am, noon and 7pm. A series of oligomers was extracted from fibers with dilute acid. In addition, a high molecular weight "glue" matrix was extracted from fibers, isolated and cleaved with either proteases or dilute acid to yield a similar series of oligomers. The cell wall subunits and "glue" matrix were found to vary both in the quantity extracted and in the character of the "glue" matrix extracted. This variability is dependent both on the time of day which the fibers were collected. The "glue" matrix appears to add carbohydrate residues to the subunits in the matrix after the initial carbohydrate residues are conjugated.