Cotton varieties containing the Bollgard™ technology have been increasing since introduction in 1996. With a growing need to combat resistant Heliothis virescens and maintain quality cotton varieties across the Mid-South, Deltapine Seed has initiated extensive grower trials to investigate and expand the number of varieties currently containing this technology. The objective was to obtain data pertinent to the growth and development of Bollgard™ cotton varieties that extended beyond sole insect control. Results from 14 on-farm trials in the South Delta of Arkansas, Louisiana and Mississippi showed that DP 20B, DP 428B, DP 32B and DPX 8C49B possessed the greatest consistency in yield, return-on-investment, earliness, fruit retention and fiber qualities. Further data also indicated that DP 20B, DP 428B and DPX 8C49B produced the greatest amount of fruit between nodes 6-10 and 11-15 indicating a tendency toward earliness and a need for adequate early season management.