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A Rapid Test for Honeydew Contamination Using the Clinitest Reagent

Roy M. Broughton, Jr. and Robert Wade Wallace


The problems associated with honeydew contamination in cotton are well documented and understood within the cotton community. We reported last year, our attempt to develop a rapid test for the detection of honeydew contamination (Topping and Broughton, 1996). The work reported this year is a continuation, a new undergraduate student project by Wade Wallace, a senior in Textile Management and Technology at Auburn. The work documents our attempt to make sure that the procedure would detect the complex sugars which are most responsible for the stickiness associated with honeydew. It was found that hydrolysis of these sugars with dilute mineral acid, at elevated temperature, for a short period of time, would allow detection by the Clinitest® (registered trademark of Miles Inc.) reagent. The acid digestion selected did not produce a detectable quantity of reducing sugars from hydrolysis of cotton.

Reprinted from Proceedings of the 1998 Beltwide Cotton Conferences pp. 1544 - 1547
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998