Prowl^® (pendimethalin) and Treflan^® (trifluralin) are the leading dinitroanilines used in the cotton industry. Over the years many studies have proven the importance of early season stand health on cotton development and yield. Rapid early root development is essential for the uptake and utilization of nutrients and systemic insecticides. "Root pruning" has long been associated with the use of DNA herbicides. This study was conducted compare the effect of the two most widely used DNA herbicides on cotton development and yield.