Experiments were conducted at Goldsboro, NC and Milan, TN to investigate weed control, cotton tolerance, and yield of new transgenic cotton varieties (Gossypium hirsutum) with a standard non-transgenic variety, Stoneville 474. The BXN variety used in the study was BXN 47 and the Roundup Ready varieties were Paymaster 1220RR and 1330RR in Tennessee and North Carolina, respectively. There were weed-free checks for the non-transgenic variety, the BXN variety, and the Roundup Ready variety at each location which allowed for direct comparisons of the yield potential. These weed-free plots were treated with Prowl at 39 fl. oz/ac plus Gramoxone at 24 fl. oz. product/ac plus Cotoran at 1.0 quart/ac applied preemergence (PRE). Plots were maintained weed free with additional hand weedings and hand hoeings as needed.