The effects of enzyme treatment on the strength and abrasion resistance, surface appearance, subjective hand qualities, and dyeing characteristics of cellulosic textiles have been widely studied and reported [Diller et. al., 94; Kumar et. al., 94; Kundu et. al., 93;]. Literature also gives significant information [Koo et.al., 94; Bhatawdikar et. al., 92; Choe et. al., 97] on how exactly the properties of cellulosic textiles are affected when they are enzyme treated before and after being subjected to other wet treatments such as desizing, scouring, bleaching, mercerization, and dyeing. However, the effects of fabric agitation on the hand related fabric properties, and the influence of these property changes on the primary hand qualities such as Koshi (stiffness), Numeri (smoothness), and Fukurami (fullness and softness) have not been widely studied and reported. Changes occurring in the thermal comfort performance of the treated fabrics have also not been fully investigated. This work reports the measured changes in the mechanical and surface properties of plain weave fabrics subjected to enzyme treatment with and without mechanical agitation. It also describes the influence of the property changes on the measured hand and appearance qualities.