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Expression of a Promoter From Afiber-specific Acyl Carrier Protein Gene in Transgenic Cotton Plants

P. Song, J. Heinen, T. Burns, K. Hisao and R. D. Allen


Based on the cDNA sequence of a fiber-specific acyl carrier protein gene isolated by differential display, the promoter sequence (Gh10) of this gene was isolated by inverse PCR. A gene cassette was constructed by combining Gh10 promoter with -glucuronidase (GUS) reporter gene and a 35s terminator. This gene construct was used to generate transgenic cotton plants by Agrobacterium mediated transformation. Transgenic plants regenerated from 3 independent cell lines were analyzed for the expression of GUS gene in different tissues and developing fibers. Histochemical staining indicated that GUS gene was strongly expressed in young developing tissue such as shoot, anther, petal, and developing fibers, but not in stripped ovules (fibers removed). GUS activity was significantly higher in fibers of transgenic plants than in nontransgenic plants. Therefore, the Gh10 promoter has the potential in the modification of cotton fiber properties through genetic engineering.

Reprinted from Proceedings of the 1998 Beltwide Cotton Conferences pp. 486 - 488
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998