We have constructed a molecular map in cotton and have identified locations of QTLs for fiber quality properties, by use of F₂ progeny from an interspecific cross between two improved cottons, G. hirsutum L. acc. TM-1, and G. barbadense L. acc. 3-79. The unique high quality fiber characteristics of 3-79 and the high productivity and wide adaptability of TM-1 led to our choice of these parents for a polymorphic mapping population. Presented here is a framework map consisting primarily of RFLP and RAPD markers, with some SSR markers, among 171 F₂ individuals of TM-1 X 3-79. These 219 loci are assembled into 40 linkage groups and cover 3,855 cM of the cotton genome. More than one half of the linkage groups have been assigned to their respective genomic origin (A vs. D) or chromosomal identity (1 through 26) by use of diploid and aneuploid cottons.