The effects of opening, carding, and repeated drawings on single fiber and bundle cotton characteristics were studied by employing Mantis^®, AFIS^® and HVI testers. Some of the significant changes in single fiber properties were found to be due to process parameters as well as the changes in the fiber crimps, parallelness of fibers within HVI beards, and the actual changes in the tensile properties of the fibers. The study showed that the HVI test data taken just prior to spinning had the highest correlation with the yarn tensile properties. Based on the study results, the authors point out the potential of HVI for future quality and process control in spinning by recommending a set of expanded HVI output that are more scientific and comprehensive for the future control needs.