Sugars occur on raw cottons through two sources. The first, normal plant sugars are part of the growing process. As many as 10 known carbohydrates have been identified to be present in normal plant sugar extracts from cotton. The most predominant of these carbohydrates are the monosaccharides glucose and fructose. The second source, honeydew (insect sugars) occurs in the form of highly sticky droplets of more complex concentrated carbohydrates on the surface of cotton. Extracts from honeydew cottons contain normal plant sugars as well as the sucrose isomer trehalulose and a trisaccharide melezitose. Work using anion High Performance Liquid Chromatography (HPLC) has made it possible to separate, characterize, and quantify these sugars. Identifying specific carbohydrates and determining their overall contributions to cotton stickiness potential is an important step in developing intervention methods to alleviate cotton stickiness experienced in textile processing. The relationship between individual sugar concentrations; specifically honeydew sugars, and the USDA reducing sugar measurement is explored.