Cotton Leaf Curl Virus (CLCuV) is a white fly (Bemisia tabaci) transmitted Gemini virus which caused significant losses to the cotton crop during the last three years in Pakistan. Production was reduced to 8.5 million bales in years1992-93 TO 1996-97 from 12.8 million bales in 1991-92. At present no virus resistant variety is available for commercial cultivation. Genetic Engineering approaches are being employed to develop transgenic CLCuV resistant plants. The CLCuV has been characterized and antisence viral constructs were made. Efforts are under way to develop transformation protocols for Pakistani cotton varieties. The present work is an attempt to develop these protocols which would be used for producing CLCuV resistant cotton plants in Pakistan.