Timely, consistent, and vigorous cotton seedling establishment is critical in establishing yield potential in cotton production. In many areas, including West Texas, unfavorable environmental conditions often exist during the optimum planting and early season growth periods. Thus, management practices which would enhance seedling establishment and early season growth would be beneficial to profitable cotton production. This study was conducted to evaluate the effects of seed applied Plant Growth Regulators (PGRs) on cotton germination, emergence, and growth. Nine commercially available materials (Arise, Cytoplex, Early Harvest, Maxon, PGR-IV, Pix, Ryzup, Stimulate, and Triggrr were applied to seed at 0.5x, 1.0x, and 2.0x the recommended rates. No significant increases in germination or emergence were noted due to the PGR treatments when compared to the control. Ryzup provided significant increases in plant height in the growth chamber at all three rates, when measured at 15 days after planting. Ryzup also increased plant height in the field at the 1.0x and 2.0x rates, when measured at 14 days after planting.