Antibiosis of ten cotton genotypes to the cotton leaf worm Alabama argillacea, was studied in laboratory. Antixenosis was determined releasing 50 moths inside field cages containing plants of each genotype potted individually. Larvae fed on genotypes 2 (Guazuncho 2 INTA) and 10 (Bulk population T94) showed the smallest numbers in larval weight, pupal weight and time to pupation. Genotypes 6 (nectariless), 5 (glabrous), 7 (glabrous and okra leaf) and 4 (glandless) were the least preferred for oviposition. These results represent preliminary studies toward a continuous characterization of future genotypes registrations in Argentina.