Sixty cotton fields were surveyed representing fifteen counties in the non-delta region of Mississippi for plant-parasitic nematodes associated with cotton production. A total of ten genera of plant-parasitic nematodes were identified. Root-knot nematodes, (Meloidogyne spp.), were identified in 10% of sites sampled, reniform nematodes, (Rotylenchulus reniformis), were identified in 15% of sites sampled, and lance nematodes, (Hoplolaimus spp.), were identified in 40% of sites sampled.