The efficacy of jojoba oil against different stages of the phytophagous mite, Tetranychus arabicus Attiah, and the adult female of the predaceous mite Euseius scutalis (Athias-Henriot) was studied. Predaceous mite females were more tolerant to the oil than any of the phytophagous mite stages, where the LC₅₀ and LC₉₀ for E. scutalis adult females were 5.08 and 14.36 %, while those for T. arabicus larvae, deutonymphs, adult females and eggs were 0.53 and 4.28, 1.21 and 5.17, 1.60 and 6.35, and 2.53 and 10.86 %, respectively. The biological aspects of T. arabicus were highly affected by treating eggs and adult females with different concentrations of jojoba oil. In egg treatment, hatchability increased while incubation period of eggs as well as life cycle were prolonged as concentration increased, and the percentage of individuals which succeeded to reach adulthood was affected. After adult treatment, life cycle was prolonged and the female fecundity decreased as concentration increased. The female longevity was prolonged in case of the low concentration (1.5 %) and was shortened in case of the high concentration (2 %). A greenhouse experiment was conducted to evaluate jojoba oil for control of the two-spotted spider mite, T. arabicus, population with three concentrations. The percentages of reduction one, 3, 7 and 15 days after treatment were 38.13, 51.12, 56.87 and 63.19 % with the oil concentration of 1 %; 60.50, 70.50, 79.30 and 80.20 % with 2 % and 76.26, 78.81, 92.66 and 92.44 % with the concentration of 3 %, respectively.