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Investigation of Transferring the Bar Gene into Cotton Via the Pollen-Tube Pathway

S.H. Moore, T.P. Croughan, G.O. Myers, and P.R. Vidrine


 
ABSTRACT

Foreign genes are often inserted into cotton DNA through the use of a "gene gun" which propels the gene construct into the protoplasm. The technique studied here attempts to transfer genes in solution through pollen tubes. Solutions containing the BAR gene for Glufosinate (‘Ignite' or ‘Liberty') resistance, coupled to an actin promoter, were applied to severed styles of flowers from cotton in the summer of 1995. Harvested seed were planted in the greenhouse and young plants were sprayed with ‘Liberty' (Glufosinate) herbicide. Cotton plants were decisively killed by the application of Liberty herbicide, indicating that the gene was not successfully incorporated into the DNA to provide resistance. The idea of making genes physically present within ovules during fertilization is appealing and perhaps more refinement or attempts are needed to obtain success.



Reprinted from Proceedings of the 1997 Beltwide Cotton Conferences pp. 483 - 484
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998