In order to be commercially acceptable, pest resistant cultivars must combine high lint yield and fiber quality with pest resistance properties. The task of combining yield, fiber quality, and pest resistance would be facilitated by information on the breeding value of available pest resistant genotypes. The objective of this study was to identify pest resistant genotypes that have high general combining ability (GCA) with adapted Midsouth cultivars. Two groups of pest resistant genotypes were evaluated for GCA using a line by tester scheme. In Group 1, DES936 and ‘Stoneville 907' had the highest GCA for lint yield. In Group 2, WC22NSS (developed in Arizona) and MAR NLBCDCAG8S-2-90 (developed in Texas) had the highest GCA for lint yield. Findings from Group 2 suggest that utilizing germplasm from outside the Midsouth may be beneficial to breeders seeking to increase yield as well as incorporate pest resistance traits into high yielding backgrounds.