Recently, efforts by our laboratory have been focusing on improving the market value of the various cottonseed products. To advance this effort, we initially have focused on the development of a rapid and an efficient plantlet regeneration system and, subsequently, the coupling of this system to Agrobacterium-mediated transformation (Figure 1) (GUS Gene Fusion System Kit). This abstract will focus on the characterization of gene transfer (NPT II & b-glucuronidase/GUS) from Agrobacterium to various tissues of cotton.