ABSTRACT
Aside from the narrow range of genotype-specificity of creating transformed plants through somatic embryogenesis, plants regenerated in culture through tissue dedifferentiation, harbor silent genetic mutations which result in lowered yields. A method based on inoculation of isolated seedling shoots with the biological vector Agrobacterium tumefaciens, overcomes these problems (Gould & Smith, 1988; Gould et al., 1991a; Gould et al, 1991b; US Pat. 5,164,310). This method was used to generate putative transgenic plants of Gossypium hirsutum: Acala SJ-2, Tamcot HQ95, Tamcot Sphinx, Stovepipe, CA 3076, CA 3085, and 91D-92 which were fertile and produced R1, R2, and R3 progeny carrying the transferred genes.
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