Verticillium wilt occurs throughout the U.S. and causes yield losses in cotton (Gossypium hirsutum). Yield losses in Arkansas due to Verticillium wilt were estimated to be 3% in 1995. Disease symptoms in the field generally occur later in the growing season and the pathogen (Verticillium dahliae) is usually not uniformly distributed in a field. An effective method for inoculating and evaluating seedlings for resistance would be useful. The objective of this study was to evaluate several methods of inoculating seedlings with V. dahliae.