In 1994, cotton (Gossypium hirsutum L.) bolls of two cultivars, DES 119 and Deltapine 90, were hand harvested by fruiting sites to compare their fiber properties. The two cultivars were grown in field plots in a randomized complete block design with four replications. All plants from a 10-foot section of row were box mapped in each of the four replications. The cultivars were grown under normal growing conditions with good insect control. Fiber properties of the cultivars differed significantly. Deltapine 90 fibers were stronger, shorter, more mature, flatter, and had a smaller perimeter than DES 119. Fibers that developed in early season were more mature and had a higher micronaire. Fiber from all positions of the upper nodes was stronger than fiber from middle and lower fruiting sites. First position fibers were significantly longer than third position fibers. Mature fibers were more ribbon like and had greater wall thickness when compared to immature fibers. Seed size decreased and lint percentages increased at the upper nodes. DES 119 and DP 90 produced yields of 1390 and 1441 pounds of lint per acre, respectively. Bolls from DES 119 and DP 90 from positions 1, 2, and 3 respectively produced 78 vs. 73%, 19 vs. 21%, and 4 vs. 6% of the total yield.