The increased concentration of poultry industries in certain parts of the Southeastern United States is leading to serious poultry waste disposal problems. As part of on-going safe litter disposal studies, fresh and composted poultry litter with and without a nitrification inhibitor, carboxymethyl pyrazole (CMP), were applied to cotton (Gossypium hirsutum cv DPL-51) to provide 45N, 90N, or 135N kgha^-1. The study was conducted in 1994 at the Tennessee Valley Sub-station, Belle Mina, Alabama. All sources of nitrogen increased cotton lint yields significantly from 1280 kgha^-1 in the control plot. CMP had no effect on cotton yield. Fresh poultry litter (1650 kgha^-1) improved cotton yields significantly more than urea (1520 kgha^-1) or composted poultry litter (1520 kgha ^-1). These results indicate that fresh poultry litter can be substituted for inorganic N sources in cotton production in the Tennessee Valley Region.