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Cloning and Characterization of Cotton Fiber Genes Encoding Lipid Transfer Protein

H.C. Liu, D.P. Ma


A fiber-specific cDNA clone (GH3) encoding lipid transfer protein (LTP) was used as a hybridization probe to retrieve corresponding genomic clones from a cotton genomic library. A LTP genomic clone (GH6) was isolated and its open reading frame (ORF) is homologous to that of GH3, but is interrupted by a single intron (136 bp) located in the region corresponding to the C-terminal of the protein. A long distance PCR method was used for 5' genomic DNA walking to clone promoter and upstream regulatory elements of the GH3 Ltp gene.

Reprinted from Proceedings of the 1996 Beltwide Cotton Conferences pp. 1171 - 1172
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998