A fiber-specific cDNA clone (GH3) encoding lipid transfer protein (LTP) was used as a hybridization probe to retrieve corresponding genomic clones from a cotton genomic library. A LTP genomic clone (GH6) was isolated and its open reading frame (ORF) is homologous to that of GH3, but is interrupted by a single intron (136 bp) located in the region corresponding to the C-terminal of the protein. A long distance PCR method was used for 5' genomic DNA walking to clone promoter and upstream regulatory elements of the GH3 Ltp gene.