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Response of an Animal Model to Mixtures of Endotoxin and N-Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Aerosols

D.G. Frazer, V.A. Robinson, P.D. Siegel, N. Al-Humadi, A.A. Afshari, W.T. Goldsmith, S. Olenchock, M.P. Whitmer, V. Castranova


 
ABSTRACT

Since both endotoxin and FMLP have been identified in cotton dust, it is important to examine how the interaction of these two agents may contribute to the biological response attributed to cotton dust. The object of this study was to determine how the simultaneous exposure to FMLP and endotoxin differed from the exposure to FMLP or endotoxin alone in the guinea pig animal model. Results showed that simultaneous exposure to the two agents caused a reduction in the pulmonary cellular response in terms of total cells, granulocytes and lymphocytes recovered from the lungs by broncho-alveolar lavage when compared with the response to endotoxin alone. The breathing rate response measured in 10% CO2 in air of guinea pigs exposed to a mixture of endotoxin and FMLP was more like animals exposed to FMLP alone than to animals exposed to endotoxin alone. In contrast, the macrophages from animals simultaneously exposed to FMLP and endotoxin appeared to have been activated to a greater extent than macrophages from animals exposed to either FMLP or endotoxin alone. These results are consistent with the hypothesis that macrophages activated by a combination of FMLP and endotoxin are inhibited from either producing or releasing factors which contribute to the cellular infiltration into the lung that normally follows endotoxin exposure.



Reprinted from Proceedings of the 1996 Beltwide Cotton Conferences pp. 360 - 363
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998