Rapid Methods of Infesting Cotton Plants with Pink Bollworm Eggs

F. Douglas Wilson and Jayne L. Szaro


 
ABSTRACT

Plants of cotton, Gossypium hirsutum L., must be infested artificially with insect eggs or larvae to evaluate them specifically for antibiosis because this method ensures uniform infestation and bypasses any differences in oviposition that could occur. Previous methods of infesting plants with pink bollworm, Pectinophora glossypielia(Saunders), involved tagging flowers and then infesting bolls of known age with eggs or larvae. These methods worked well because the larvae were able to penetrate the bolls and develop in the seeds as they would have if the moths had laid the eggs on the bolls. However, these methods are too slow to be useful in large field experiments where several thousand plants must be evaluated. Recently, we have developed two rapid methods. For the first one we use an automatic repeating pipette to infest leaf axils and plant terminals with eggs, and depend upon the first instar larvae to migrate to the bolls. This method worked well in the greenhouse but not in the more stressful field environment. For the second method, we sprayed an egg/gum xanthan solution in distilled water on the underside of leaves and other parts of the plant, using a spray wand attached to a spray apparatus that ensures a constant pressure flow. This method has also worked well in the greenhouse but has not yet been tested in the field. We predict that it will be more satisfactory than the pipette method because at least some eggs will be sprayed directly on the bolls and many will be closer to the bolls than those sprayed on leaf axils or terminals. Also, the eggs should be protected more from elements because they will be placed on shaded portions of the plant.



Reprinted from 1986 Proceedings: Beltwide Cotton Production Research Conferences pg. 493
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998